WebJul 27, 2024 · 实验步骤 组织破碎裂解,提取蛋白和RNA混合物;磁珠、抗体、蛋白和RNA混合物过夜孵育;多次洗涤,除去非特异结合的RNA;解交联,纯化RNA片段;q-PCR或测序分析。 案例分享 The transcription factor CBFB suppresses breast cancer through orchestrating translation and transcription (Nature Communications .2024). CBFB基因编 … WebJan 27, 2024 · In PAR-CLIP, cells are incubated prior to crosslinking with modified nucleosides, 4-thiouridine (4SU) or 6-thioguanisine (6SG) that get incorporated into nascent RNAs. The exocyclic thione group increases the photoreactivity of the base, allowing lower energy UV light (312 ≤ λ ≤ 365 nm) to be used for crosslinking.
RNA结合蛋白与RNA相互作用鉴定技术 - bjmu.edu.cn
Web在PAR-CLIP方法中,活体细胞被暴露在紫外灯下,使得RNA-蛋白结合起来并且可以用免疫共沉淀的方法使特异性的蛋白以及与其结合RNA一同被分离出来。 这个方法中最重要的一点就是方依赖了具有光活性的核糖核苷类似物,例如4-硫尿核苷,在活体细胞中将其插入到新生RNA转录本中。 在356nm紫外灯辐射的细胞中,光活性的核糖核标记的RNA被诱导 … Web手把手教你玩转“面包板”,详解了面包板结构,实际使用案例,用面包板做了电容串联和并联实验。, 视频播放量 59365、弹幕量 90、点赞数 1697、投硬币枚数 533、收藏人数 774、转发人数 377, 视频作者 多多百科, 作者简介 电子知识科普,电子diy,航模制作,单片机,编程 … how to delete a township tale server
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WebMay 25, 2024 · 为了避免类似情况,请为“ 科研人直通车 ”设置 星标 ,文章点一下“ 在看 ”,保持互动热度,就能及时收到每期推送啦!. CoIP :实验原理. 要守护爱情,先了解如何相处,一切从 CoIP 的原理谈起:免疫共沉淀是一种以抗体和抗原识别专一性为基础,用于研究 ... PAR-CLIP (photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation) is a biochemical method for identifying the binding sites of cellular RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs). The method relies on … See more • CLIP-Seq, a similar method for identifying the binding sites of cellular RNA-binding proteins (RBPs) or RNA modification sites using UV light to cross-link RNA to RBPs without the incorporation of photoactivatable … See more • starBase database: decoding miRNA-mRNA, miRNA-lncRNA, miRNA-sncRNA, miRNA-circRNA, miRNA-pseudogene, protein-lncRNA, protein-ncRNA interactions and ceRNA networks from PAR-CLIP(CLIP-Seq, HITS-CLIP,iCLIP) data, and See more the moors homeowners association