Camptothecin hl-60

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WebThis potent antioxidant is a cytoprotectant in several experimental models. However, its neuroprotective activities are scarcely available, especially in the hippocampal region associated with... WebAug 1, 1999 · HL-60 human promyelocytic leukemia cells were cultured in RPMI 1640 supplemented with 10% fetal bovine serum. For induction of apoptosis, cells were exposed for 3 h to 0.1 μg/ml DNA topoisomerase I inhibitor, camptothecin. Quantitative analysis of apoptosis was carried out by flow cytometry.

There is substantial nuclear and cellular disintegration before ...

WebMay 1, 2000 · The promyelocytic HL-60 cell lines have been used extensively to study drug-induced apoptosis, particularly with camptothecin (Palissot et al., 1996; Shimizu and Pommier, 1997; King et al., 2000 ... WebWe investigated expression of cyclin A in HL-60 cells after induction of apoptosis with doxorubicin and etoposide. Following apoptotic trigger, both cells arrested in G2/M phase of the cell cycle... literary terms review scavenger hunt

There is substantial nuclear and cellular disintegration before ...

WebThe Aerospace Maintenance Competition is an annual celebration of aviation maintenance technicians and their skills. Held during Aviation Week at the MRO Ame... WebFounded. 1932. Founder. Harold L. Green. Defunct. 1990s. Fate. Closed as part of parent company ( McCrory Stores) bankruptcy in 2001. H. L. Green was a five and dime store … WebSep 1, 2001 · To shed light on the mechanism of action of hCPT at the cellular level, we compared the effects of BN80915 and the classic camptothecin SN-38, the active metabolite of irinotecan, on HL-60 human promyelocytic cancer cells. important events in u s history

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WebJan 15, 2024 · In summary, 12 novel biotinylated camptothecin derivatives were designed, synthesized, and evaluated for anticancer activities against a panel of five human cancer … WebJun 1, 2000 · Camptothecin (50 mM), a cytotoxic quinoline alkaloid which inhibits the DNA enzyme topoisomerase I, was used as a positive control (King et al., 2000). ... Internalization of Bacillus intermedius... important events in the paleozoic eraWeb14808-60-7 Wt. % 60 - 100 1 - 5 This product is a hazardous chemical as defined by the OSHA Hazard Communication Standard, 29 CFR 1910.1200. Gypsum (calcium sulfate) … literary terms scavenger hunt

"WebDNA single- strand breaks were detected using alkaline sucrose gradient centrifugation when HL-60 cells were incubated with 10 μMCPT or 10 μMSN-38 for 30 min. These … " - Camptothecin hl-60

Camptothecin hl-60

WebCamptothecin, a cytotoxic plant alkaloid with antitumor properties, is a prototypic DNA topoisomerase I inhibitor. ... This camptothecin concentration range works well for inducing Jurkat or HL 60 cell suspensions which usually run in the 1 x 105 – 1 x 106 cell/mL concentration range. At this concentration range successful apoptosis induction ... WebNational Center for Biotechnology Information

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WebNov 1, 2002 · The role of mitochondria in HL-60 apoptosis was explored using an inhibitor of oxidative phosphorylation, antimycin A (AMA). Methods: Changes in cell light scatter, … WebCamptothecin is a plant alkaloid present in wood, bark, and fruit of the Asian tree Camptotheca acuminata. From a shaky beginning complicated by toxic effects and logistical issues, camptothecin derivatives have advanced to become standard components in the treatment of several malignancies.

WebThe meaning of CAMPTOTHECIN is an alkaloid C20H16N2O4 from the wood of a Chinese tree (Camptotheca acuminata of the family Nyssaceae) that has shown some … WebJul 26, 2024 · CP6 displayed potent and comparable cytotoxicity. Interestingly, CP6 exhibited weak cytotoxicity against the normal cell line HUVEC and HL-7702 with a higher IC 50 (22.5 μM) and (18.5 μM). CP6 show minimal cytotoxicity against the human cervix adenocarcinoma cancer cell line HeLa with an IC 50 of 5.5 μM.

WebCamptothecin is a naturally occurring alkaloid derived from the plant Camptotheca acuminata that was identified in an anticancer drug discovery screen in the 1960s. 68 … WebJun 13, 2024 · Camptothecin is an anticancer molecule which acts by targeting the human deoxyribonucleic acid topoisomerase I (Topo-I). It inhibits the enzyme by blocking the re-joining step of the cleavage reaction of Topo-I, which causes accumulation of a covalent reaction intermediate.

WebJun 29, 2015 · ；D. 大黄素无法捕获Topo I-DNA 的可切割复合物，Topo 的抑制剂CPT作为阳性对照；大黄素能够诱导稳定的TopoII α-DNA 可切割复合物，Topo II 的抑制剂VP16 作为阳性对照。G. 用大黄素处理HL-60 和HL-60/MX2 细胞后，检测γ -H2AX 的表达。H. 大黄素和VP16在Topo II –缺陷型细胞HL ...

WebAug 1, 1993 · Since apoptosis has been associated with potential involvement of intracellular signaling linked to the Ca 2+ /calmodulin and protein kinase C transduction … important events in uaeWebThere have been some conflicting results concerning whether annexin V binds to camptothecin (CAM)-treated HL-60 cells, a commonly used model for apoptosis. We investigated the effects of culturing HL-60 cells for up to 8 h with a range of CAM concentrations. METHODS We used flow cytometry to measure cellular light scatter, … literary terms practice worksheetsWebCamptothecin is a naturally occurring alkaloid derived from the plant Camptotheca acuminata that was identified in an anticancer drug discovery screen in the 1960s.67 … important events in u.s historyWebOct 1, 2001 · The study identifies and delineates signaling factors involved in BN80915-induced apoptosis in HL-60 cells. Cell cycle analysis of HL-60 cells treated with graded … literary terms starting with hWebHL-60 cells exposed to okadaic acid and camptothecin underwent morphological and biochemical changes typical of apoptosis, including internucleosomal DNA fragmentation and PAI-2 cleavage. literary terms starting with b important events in westward expansionWebAug 26, 2002 · Camptothecin (CAM; Sigma, St. Louis, MO) initiates apoptosis in the human promyelocytic leukemia cell line, HL-60, via its binding to topoisomerase I–DNA … literary terms point of view